MGI Tech DNBSEQ-G400RS FluoXpert multi-omics instrument

MGI Tech’s DNBSEQ-G400RS FluoXpert is a multifunctional sequencer that is compatible with in vivo spatial protein detection. Integrating high-throughput sequencing and multiplex immunofluorescence (mIF) staining, the fully automated, high-plex, multi-omics instrument combines DNA/RNA and protein analysis in one, offering a powerful upgrade from conventional pathology and spatial proteomics tools.

The device integrates optimised hardware, state-of-the-art biochemistry and innovative software for an all-in-one multi-omics experience. A comprehensive and versatile benchtop sequencer is equipped with optimised optical, mechanical, fluidics and electrics systems, empowering a rapid and streamlined sequencing process.

The platform also comes with the flexible and easy-to-use FluoXpert mIF reagent set. It is compatible with both frozen tissue and FFPE samples, as well as off-the-shelf antibodies, eliminating the need for a complex conjugation process. Available in standard and customised options, it is backed by an autofluorescence quenching reagent and a mild but efficient elution technology, for low residual signal and optimal antigen preservation.

The FluoXpert program supports a fully automated process of fluorescence staining and high-resolution scanning and imaging. Equipped with a customisable protocol, it provides real-time image visualisation, antibody library management, and independent wash and elution. The resulting images are high in quality, with seamless stitching and 20x subcellular resolution (360 nm per pixel). Due to the efficient fluid exchange and mild elution reagents, sample tissue remains intact despite multiple rounds of staining, for robust reproducibility.

At 50 min/cycle, the product is designed to triple the speed of conventional manual staining and imaging, making it suitable for multiple staining cycles. Its optimal throughput ranges from 20 to 40 sections per week on the standard 6-plex staining kit, while the highest throughput can reach up to 140 sections per week on the same kit. It demonstrates good performance in spatial protein identification, making it suitable for use in different fields of proteomics research.