Revvity Dharmacon ON-TARGETplus siRNA for precision gene knockdown

Dharmacon ON-TARGETplus siRNAs by Revvity combine a patented dual-strand modification pattern with the SMARTselection algorithm to deliver efficient and specific gene silencing, making the product suitable for RNAi experiments.

Benefits are said to include: up to 90% reduction in off-target effects compared to unmodified siRNA; guaranteed target silencing, with at least three of four individual siRNAs in each SMARTpool performing effectively; and full sequence information provided with every siRNA purchase for transparency and reproducibility.

The siRNAs are available for human, mouse and rat targets, with users able to choose the format that best fits their workflow. SMARTpool is a potent and specific mixture of four siRNAs combined in one reagent for good knockdown. Alternatively, users can purchase aliquots of four individual siRNAs targeting their gene of interest. Single siRNAs can also be selected for targeted experiments.

The siRNAs are designed to give researchers the confidence to interrogate gene function with precision and specificity. Experimental success can be maximised by pairing ON-TARGETplus with Dharmacon non-targeting and positive controls, along with DharmaFECT transfection reagents or electroporation for efficient intracellular delivery.