Antibody screening

Monday, 13 November, 2006 | Supplied by: http://www.pathtech.com.au/


The Guava Technologies RapidQuant IgG kits are a line of quantitative bead-based kits designed to quantify either mouse or human IgG antibody (Immunoglobulin G) during hybridoma screening.

The kits represent a new approach to antibody quantitation by allowing assessments to be easily performed on a plate-based benchtop flow cytometer instead of traditional plate readers. Available for both human and mouse antibodies, the kits have simplified protocols that require very little sample manipulation and significantly less time than traditional ELISA protocols. The kits are optimised for easy use on the Guava EasyCyte system.

With the RapidQuant IgG kits, users can easily compare IgG production on large numbers of hybridoma clones using very small volumes of supernatant. The kits combine with other Guava assays to accelerate the entire process of antibody screening by allowing all stages of screening to be performed from the culture media in a single well, typically without having to expand clones.

The RapidQuant IgG assay provides rapid, reproducible and accurate results in the most appropriate range for screening hybridoma culture media. Designed as universal IgG kits, the RapidQuant mouse IgG and human IgG assays quantify all IgG subtypes equivalently, saving time with a single assay.

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