Mutliphoton imaging

The FluoView FV1000MPE microphoton laser scanning microscope system offers bright clear imaging in living cells and deep within specimens.

With four base models with flexible configurations available, researchers can obtain a system that best suits their needs now and in the future. The systems are suitable for neuroscientists and biologists who need to image dynamic processes up to several hundred microns deep in living specimens, with minimal bleaching or damage.

At the core of the FV1000MPE technology is the optical design. An acousto-optic modulator (AOM) adjusts laser light intensity, with rapid on/off switching. This is combined with an automatic beam expander that maintains maximal filling of the entry pupil of the microscope objective when changing excitation wavelengths and objectives.

The system features a multiphoton-dedicated 25x objective (the XLPLN25XWMP) with a correction collar that can be adjusted to compensate for any refractive index mismatch, allowing the formation of a focal spot deep within the sample without reducing energy density.

Olympus incorporates a widefield design into both the FV1000MPE detectors and XLPLN25XWMP objective, to capture the maximum amount of fluorescence signal for imaging. A transmitted fluorescence detector with a dedicated NA condenser allows for extremely bright fluorescence imaging deep within a live specimen, and also allows concurrent second harmonic generation (SHG) imaging.