Clonetics Prostate Smooth Muscle Cell Systems (PrSMC)

Capsugel Australia Pty Ltd
Friday, 15 October, 2010


Introduction

Clonetics Prostate Smooth Muscle Cell Systems contain Normal Human Prostate Smooth Muscle Cells (PrSMC) and optimized medium for their growth. Each System can generate PrSMC cultures for experimental applications in benign prostatic hyperplasia (BPH), prostitis and prostate cancer.

Clonetics Prostate Smooth Muscle Cell Systems are convenient and easy to use, allowing the researcher to focus on results. Cryopreserved PrSMC are shipped in second or third passage.

Proliferating PrSMC are shipped in third or fourth passage.

Clonetics Cells, Medium and Reagents are quality tested together and guaranteed to give optimum performance as a complete cell system.

Cell System Components

  • One Prostate Smooth Muscle Cell Product (Cryopreserved or Proliferating)
  • One Smooth Muscle Cell Medium BulletKit - 500 ml Clonetics SmGM-2 BulletKit (CC-3182) contains one 500 ml bottle of Smooth Muscle Cell Basal Medium and the following growth supplements: hEGF, 0.5 ml; Insulin, 0.5 ml; hFGF-B, 1ml; FBS, 25 ml; GA-1000, 0.5 ml.
  • One ReagentPack (CC-5034) Containing: Trypsin/EDTA 100 ml Trypsin Neutralizing Solution 100 ml HEPES Buffered Saline Solution 100 ml

Characterization of Cells

Routine characterization of PrSMC includes immunofluorescent staining. Cells stain positive for smooth muscle a-actin and negative for von Willebrand (Factor VIII) antigen.

Performance

Recommended seeding density for subculture 3,500 cells/cm2

Typical time from subculture to confluent monolayer 6 - 10 days

Additional population doublings guaranteed using Clonetics System 10

Quality Control

All cells are performance assayed and test negative for HIV-1, mycoplasma, Hepatitis-B, Hepatitis-C, bacteria, yeast and fungi. Cell viability, morphology and proliferative capacity are measured after recovery from cryopreservation. Clonetics Media are formulated for optimal growth of specific types of normal human cells. Each lot of medium is tested for the support of cell viability and proliferative capacity. Certificates of Analysis (CoA) for each cell strain are shipped with each order. CoA’s for all other products are available upon request.

Ordering Information

Cryopreserved Cells

CC-2587 PrSMC 500,000 cells

Proliferating Cells - Flasks and Multiwell Plates

CC-2587T25 T-25 Flask

CC-2587T75 T-75 Flask

CC-2587W96 96-well Plate

Other proliferating formats are available. Contact Technical Service or refer to the Lonza website for details.

CC-3182 SmGM-2 BulletKit, SmBM plus SingleQuots of growth supplements 500 mL

CC-3181 SmBM, Smooth Muscle Cell Basal Medium 500 ml

CC-4149 SmGM-2 SingleQuots, Formulates SmBM to SmGM-2

CC-5034 ReagentPack Trypsin/EDTA Solution 100 ml

Trypsin Neutralizing Solution 100 ml

HEPES Buffered Saline Solution 100 ml

Product Warranty

CULTURES HAVE A FINITE LIFESPAN IN VITRO. Lonza guarantees the performance of its cells only if Clonetics Media and Reagents are used exclusively, and the recommend protocols are followed. The performance of cells is not guaranteed if any modifications are made to the complete Cell System. Cryopreserved PrSMC are assured to be viable and functional when thawed and maintained properly.

THESE PRODUCTS ARE FOR RESEARCH USE ONLY. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro procedures.

WARNING: CLONETICS AND POIETICS PRODUCTS CONTAIN HUMAN SOURCE MATERIAL, TREAT AS POTENTIALLY INFECTIOUS. Each donor is tested and found non-reactive by an FDA approved method for the presence of HIV-I, Hepatitis B Virus and Hepatitis C Virus. Where donor testing is not possible, cell products are tested for the presence of viral nucleic acid from HIV, Hepatitis B Virus, and Hepatitis C Virus. Testing can not offer complete assurance that HIV-1, Hepatitis B Virus, and Hepatitis C Virus are absent. All human sourced products should be handled at the Biological Safety Level 2 to minimize exposure of potentially infectious products, as recommended in the CDC-NIH Manual, Biosafety in Microbiological and Biomedical Laboratories, 1999. If you require further information, please contact your site Safety Officer or Technical Services.

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