Lonza EasyDiff supplements for bone marrow CD34+

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Primary human bone marrow hematopoietic progenitors are multipotent cells that can differentiate into several lineages. EasyDiff supplements allow differentiation of CD34+ cells into three different hematopoietic lineages (myeloid, erythroid and megakaryocytic) in 96-well plates.

Users simply add the appropriate volume of media (LGM-3 or IMDM) to dilute the supplement and mix with CD34+ cells in media. Once plated into 96-well plates differentiation will be complete in 10-12 days.

There is no need for methylcellulose or any substrate. Users can verify differentiation via quantification of the expression of lineage-specific markers.

The supplements are suitable for both drug discovery and toxicology research.