ProImmune ProVE SL Self-Loading MHC Class I Monomers

ProImmune has announced the launch of its ProVE SL Self-Loading MHC Class I Monomers. The novel reagents for antigen-specific CD8⁺ T cell detection are designed to offer researchers good flexibility, speed and MHC allele-coverage, with over 50 HLA alleles available.

Supplied as highly robust, empty, biotinylated MHC class I molecules, the extended allele range includes HLA-A, HLA-B, HLA-C and non-canonical alleles, as well as mouse H-2. The ProVE SL platform allows researchers to load their own peptides and flexibly combine monomers with a wide range of avidin or streptavidin conjugates — including fluorescent, barcoded or mass-cytometry reagents — to easily create tetramers or surface-bound complexes. This flexibility enables fast experimental set-up, reconfiguration of panels, expanded donor-cohort coverage — including less common alleles — and integration with advanced flow and mass cytometry or single-cell RNA sequencing workflows.

T cell immunology, epitope mapping and immune-monitoring workflows increasingly demand reagents that permit rapid customisation across diverse HLA contexts. The range addresses key limitations of conventional pre-loaded MHC monomers, which often restrict peptide choice, require complex loading and exchange steps, or cover only frequent alleles. By enabling self-loading of peptides, the ProVE SL platform empowers immunologists to adapt their reagents more quickly to new antigens, for example for infectious diseases, cancer neo-antigens, autoantigens, and measuring vaccine responses and to study donor cohorts with rarer HLA types.