Tomocube HT-X1 Holotomography System

Monday, 01 July, 2024 | Supplied by: SciTech Pty Ltd

Tomocube HT-X1 Holotomography System

The Tomocube HT-X1 optical holotomography microscope uses incoherent light to generate holographic images of unlabelled live cells. The technology unlocks label-free 3D and 4D live cell imaging on standard imaging plates for higher-throughput and automated screening applications.

Core imaging facilities will benefit from the integrated gassed incubator for long-term, time-lapse studies and the software-driven approach, allowing multiple users to access its performance simply and quickly through GUI, TomoStudio X. It has the ability to image 24-, 12- and 6-well plate formats and has a large field-of-view, laser autofocus system and a high-performance 40x 0.95NA objective.

The microscope uses optical diffraction technology (ODT) to produce morphological, mechanical and chemical properties of individual living cells through the 3D refractive index (RI) tomograms quickly and simply without any sample preparation, and molecular specificity information through fluorescence imaging.

It uses a conventional single-beam 450 nm white light LED instead of a laser light source. Instead of rotating the laser beam to illuminate the sample at various incidence angles it illuminates the sample with various beam patterns specifically designed to retrieve the refractive index and captures a sequence of holograms from different positions. This single-beam technique simplifies the imaging process by eliminating the need for background calibration and allowing imaging in confluent samples without an increase in light intensity. As well as being easily combined with complementary imaging modalities, it is designed to be mechanically more stable and less sensitive to speckle noise for high contrast imaging.

Online: www.scitech.com.au
Phone: 03 9480 4999
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