Beckman Coulter Life Sciences CytoFLEX nano flow cytometer

The CytoFLEX nano flow cytometer, from Beckman Coulter Life Sciences, is described by the company as the first purpose-built nanoscale flow cytometer for research use only that enables detection to at least 40 nm, enabling 30–50% more data creation compared to current offerings.

With high sensitivity to detect, count and characterise nanoparticles using a single analytical instrument, the flow cytometer offers simultaneous multiparameter detection powered by six fluorescent detection channels and five side scatter channels. It thus lowers the limit of detection and increases resolution to characterise lower abundance targets in heterogeneous extracellular vesicle (EV) populations between 1 μm and 40 nm (polystyrene when triggering on violet side scatter).

Nanoparticles are introduced in a hydrodynamic focused stream to the optical system composed of lasers and detectors. Emitted signals are collected, digitised and displayed by the electronics and instrument control software, CytExpert. Multiple automatic onboard cleaning options are offered to control sample-to-sample carryover and maintain a clean sample line.

The ergonomic design is intended to reduce noise, identify backgrounds and reduce carryover issues. Consistent instrument performance from extensive automated QC processes enables consistent and reproducible results.

The platform also includes the CytoFLEX, CytoFLEX S and CytoFLEX LX flow cytometers, along with the CytoFLEX SRT cell sorter for research and the DxFLEX flow cytometer for diagnostic use.