MP Biomedicals Myco-Sniff-Valid Mycoplasma PCR Detection Kit

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One of the major issues in mammalian cell culture is infection due to mycoplasma contamination. It affects various cellular behaviours including metabolism, growth, viability and morphology, compromising the validity of experimental results and study data.

Up to 30–85% of cell cultures may be contaminated by mycoplasma species, so testing for mycoplasma is an essential quality control step in order to ensure correct and reproducible results. Agar cultures and DNA fluorochrome staining methods can be used for mycoplasma detection, but these methods are time-consuming (~28 days), inconsistent and difficult to interpret.

PCR-based methods have become an accepted standard protocol for mycoplasma detection to replace direct culture methods, including European Pharmacopoeia 2.6.7 and USP 63.

The Myco-Sniff-Valid Mycoplasma PCR Detection Kit has been demonstrated to be a sensitive, specific and rapid method for the detection of mycoplasma contamination in cell cultures. Using a set of primers specific for the highly conserved mycoplasma 16S-rRNA coding region including M. pneumoniae, M. arginini, M. hyorhinis, M. fermentans, M. orale and A. laidlawii, the kit is specifically designed to detect the presence of mycoplasma that might contaminate cultured cells.

The detection can be performed within 3 h with a sensitivity as low as 10 CFU/mL. An exogenous internal control is provided to control for PCR inhibition.

A positive control verifies the effectiveness of template DNA and confirms the size of PCR products for positive samples and 8-Methoxypsoralen (8-MOP) prevents cross-contamination from previous PCR products. Each kit contains 48 PCR lyophilised tubes for 20 µL reactions.

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