Lonza PPiLight inorganic pyrophosphate assay

Tuesday, 22 March, 2011 | Supplied by: Capsugel Australia Pty Ltd

Lonza PPiLight inorganic pyrophosphate assay

The PPiLight assay is a non-radioactive bioluminescent assay for the detection of inorganic pyrophosphate (PPi).

In the presence of PPi, the detection reagent catalyses the conversion of AMP to ATP. The assay uses luciferase, which produces light from the newly formed ATP and luciferin. It has a wide detection range - sensitive to 0.02 µM with a linear range to 10 µM.

It is a simple two-step assay that doesn’t require radioactive substrates, beads, detection antibodies or modified substrates. It is scalable to 96-, 384- or 1586-well formats.

Users can measure enzyme activity via pyrophosphate consumption in 1 h. Applications include; phosphotransferases, pyrophosphotases, cyclases, ligases, hydrolases and DNA polymerases.

Online: lonzabioscience.com.au
Phone: 1300 657508
Related Products

Mercodia Insulin MBeads Assay

The assay is designed to offer high accuracy by specifically measuring human insulin while...

Waters BD FACSDiscover A7 Cell Analyzer

The BD FACSDiscover A7 Cell Analyzer combines high spectral resolution and sensitivity with...

Lumiprobe CytoTracer fluorescent cell dyes

The dyes rapidly penetrate cells, form intracellular membrane-impermeable products, and deliver...


  • All content Copyright © 2026 Westwick-Farrow Pty Ltd