Lonza PPiLight inorganic pyrophosphate assay

Tuesday, 22 March, 2011 | Supplied by: Lonza Australia Pty Ltd



The PPiLight assay is a non-radioactive bioluminescent assay for the detection of inorganic pyrophosphate (PPi).

In the presence of PPi, the detection reagent catalyses the conversion of AMP to ATP. The assay uses luciferase, which produces light from the newly formed ATP and luciferin. It has a wide detection range - sensitive to 0.02 µM with a linear range to 10 µM.

It is a simple two-step assay that doesn’t require radioactive substrates, beads, detection antibodies or modified substrates. It is scalable to 96-, 384- or 1586-well formats.

Users can measure enzyme activity via pyrophosphate consumption in 1 h. Applications include; phosphotransferases, pyrophosphotases, cyclases, ligases, hydrolases and DNA polymerases.

Online: www.lonza.com
Phone: 03 9550 0883
Related Products

BioGX Xfree COVID-19 Direct RT-PCR test

The Xfree COVID-19 Direct RT-PCR test is a complete test in a single vial, lyophilised...

Bio-Rad Laboratories CFX Opus 96 and CFX Opus 384 Real-Time PCR Systems

Bio-Rad Laboratories' CFX Opus 96 and CFX Opus 384 Real-Time PCR Systems are suitable for...

PCR Biosystems NGSBIO Library Quant Kit for Illumina

The kit contains all the components necessary for sensitive quantification of DNA libraries prior...


  • All content Copyright © 2020 Westwick-Farrow Pty Ltd