Lonza PPiLight inorganic pyrophosphate assay

Tuesday, 22 March, 2011 | Supplied by: Lonza Australia Pty Ltd

The PPiLight assay is a non-radioactive bioluminescent assay for the detection of inorganic pyrophosphate (PPi).

In the presence of PPi, the detection reagent catalyses the conversion of AMP to ATP. The assay uses luciferase, which produces light from the newly formed ATP and luciferin. It has a wide detection range - sensitive to 0.02 µM with a linear range to 10 µM.

It is a simple two-step assay that doesn’t require radioactive substrates, beads, detection antibodies or modified substrates. It is scalable to 96-, 384- or 1586-well formats.

Users can measure enzyme activity via pyrophosphate consumption in 1 h. Applications include; phosphotransferases, pyrophosphotases, cyclases, ligases, hydrolases and DNA polymerases.

Online: www.lonza.com
Phone: 03 9550 0883
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