Lonza QCL-1000 endpoint chromogenic LAL assay

Monday, 21 June, 2010 | Supplied by: Lonza Australia Pty Ltd



The QCL-1000 endpoint chromogenic LAL assay is a claimed fast and quantitative endpoint assay for the detection of gram-negative bacterial endotoxin in a wide variety of samples.

The assay can be run in tubes or a 96-well microplate incubated at 37°C using a dry heat block.

Samples are mixed with the LAL reagent and a chromogenic substrate reagent and incubated for 16 minutes. The absorbance at 405-410 nm of the solutions in the tubes or microplate is then read. Endotoxin, if present, causes a yellow colour to form.

The assay has a sensitivity range of 0.1 to 1.0 EU/mL and the endotoxin concentration of unknown samples can be calculated by comparing to a standard curve.

Online: www.lonza.com
Phone: 03 9550 0883
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