The ToxiLight bioassay kit is a bioluminescent, non-destructive cytolysis assay kit designed to measure the release of adenylate kinase (AK) from damaged cells.
AK is released into culture medium when cells die. This enzyme actively phosphorylates ADP to form ATP which can then be measured using the bioluminescent firefly luciferase reaction.
There is no need for cell lysis (required by other cytotoxicity assays) so repeated samples of supernatant can be taken over time without disrupting the cells themselves. Users can perform a kinetic analysis of cell death and/or other tests on the original cells.
Its features include: single-reagent kit makes the assay very simple to perform, good sensitivity with a detection limit of 10 cells per microwell and a dynamic range of over five orders of magnitude.
The kit is suitable for batch processing in high-throughput screening applications due to extended signal stability.
Phone: 1300 657508
Lonza 4D-Nucleofector LV Unit PRO electroporation unit
Lonza's 4D-Nucleofector LV Unit PRO is a next-generation, large-scale electroporation unit...
Cytek 25-Color Immunoprofiling Assay, cFluor Reagent Kit (18C)
The Cytek 25-Color Immunoprofiling Assay has been designed and optimised to provide a turnkey...
Bio-Rad Laboratories QX Continuum and QX700 series ddPCR platforms
The latest platforms complement Bio-Rad's existing QX ddPCR portfolio and offer users...
Learn More
I am interested in Lonza ToxiLight bioassay kit.
Complete the form and a supplier representative will be in touch.
