4basebio TruePrime Single Cell Whole Genome Amplification Kit

The TruePrime Single Cell Whole Genome Amplification Kit from 4basebio uses a novel method to achieve genome amplification from single or just a few cells. Amplification of a few cells instead of one can improve total coverage breadth if the user’s experimental design allows this.

TruePrime technology is based on the combination of the recently discovered DNA primase TthPrimPol1 and the progressive and high-fidelity Phi29 DNA polymerase. TthPrimPol is a thermostable member of the recently discovered PrimPol family of enzymes. PrimPols are endowed with primase and polymerase activities. The main novelty of these enzymes is their capacity to synthesise DNA primers.

The kit uses alkaline incubation to allow cell lysis and DNA denaturation of genomic DNA with low DNA fragmentation. This results in amplified DNA with high integrity and fragment length, ensuring that most of the sequences are uniformly represented.

Suitable for next-generation sequencing, the product is insensitive to external DNA contamination and works with all types of genomes. TthPrimPol synthesises the primers for Phi29 DNA pol and there is no random extension of primer dimers. The convenient kit format enables easy handling and is said to offer reduced amplification bias in genome coverage for correct results.