Cayman Chemical oxidative damage assay kits

Cayman Chemical has a complete range of assay kits to measure direct and indirect biomarkers of oxidative damage. While direct measurement of reactive oxygen species (ROS) is preferred, indirect measurements are favoured by researchers due to the relative stability of damage markers compared to the transient ROS.

To quantify oxidative damage, it is best practice to measure multiple oxidative stress markers. It is possible to assess multiple biomarkers on the same molecule (eg, different markers of lipid peroxidation) or to examine one marker each on proteins, lipids and nucleic acids.

Cayman’s range of oxidative damage assay kits includes kits to measure the following biomarkers: ROS (and reactive nitrogen species) — extracellular H₂O₂, NO^· metabolites, in vitro NOS activity, ROS, xanthine oxidase activity; DNA/RNA damage — 8-hydroxy-2’-deoxyguanosine, 8-hydroxy guanosine; protein oxidation (and nitration): methionine sulfoxide, S-Glutathionylated proteins, S-Nitrosylated proteins, nitrotyrosine, protein carbonylation; lipid peroxidation: 8-isoprostane, DHN-MA, lipid hydroperoxides, TBARS; and antioxidants — total antioxidants, ascorbate, catalase, glutathione, glutathione reductase, superoxide dismutase and thiol.