Echelon Biosciences PIP Mass Assays

Phosphatidylinositol phosphates (PIPs) are more than just membrane components. They are vital signalling molecules within the cell that participate in a variety of biological processes ranging from membrane trafficking to inflammation and cell death.

The production of PIP₃ by class I PI3-kinases (PI3-K) is important in multiple cell signalling pathways. Typically, experiments to measure PI3-K activity have involved phosphorylation of a phosphoinositide substrate using ³²P, then extraction of radioactive products and separation using thin-layer chromatography.

Developed by Echelon Biosciences, PIP Mass Assays offer researchers a comprehensive selection of ELISA-based assays for the measurement of PIP levels from biological samples. The PIP₃ Mass Assay allows the user to determine PI3-K activity by measuring the amount of PIP₃ extracted from cells by means of standard ELISA format, eliminating the need for radioactivity, and thin-layer chromatography.

The mass assay range is for research use only.