Enzo Life Sciences PROTEOSTAT Protein refolding and aggregation sensing kit

Wednesday, 08 May, 2024 | Supplied by: Sapphire Bioscience

Enzo Life Sciences’ PROTEOSTAT Protein refolding and aggregation sensing kit provides a concerted assay format employing a fractional factorial matrix design that facilitates screening of refolding parameters for a specific protein in different buffers, and a simple, homogenous assay format for monitoring protein aggregation using a proprietary red-emitting aggregation-sensitive molecular rotor dye. Selected samples with low fluorescence readout, and thus low aggregation content, can then be validated using traditional enzyme activity-based determination of refolding success.

The kit includes a comprehensive set of optimised screening reagents and conditions that facilitate protein refolding. With the integrated workflow, users can identify conditions that can cause off-pathway protein aggregation with PROTEOSTAT dye. They can also conduct rapid microplate screening using a design of experiments (DOE)-valued approach to identify parameters that are critical to protein formulation.

Online: www.sapphirebioscience.com
Phone: 02 9698 2022
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