Harvard Apparatus 192-well DispoEquilibrium Dialyzers

Thursday, 07 October, 2010 | Supplied by: SDR Scientific

Harvard Apparatus ready-to-use, disposable 96-sample equilibrium dialysis plates are suitable for analysis of free or unbound analytes like free T4, free cortisol, free testosterone, or for protein-ligand binding studies.

The company offers 192-well DispoEquilibrium Dialyzers that provide simple and reliable methods to analyse up to 96 samples simultaneously on disposable dialysis plates.

These ready-to-use equilibrium dialysis plates are convenient and cost-effective tools for ligand binding experiments including serum protein binding, protein-drug binding, protein-protein binding and protein-DNA binding assays.

Equilibrium dialysis is recognised as the Gold Standard or the most preferred technique for molecular binding studies as it allows for the direct assays of molecular interactions at close to physiological conditions with minimal effects on equilibrium parameters.

Equilibrium dialysis has also shown itself to be a valuable technique for sample preparation before candidate analysis by mass spectrometry. Studies have shown enhanced target identification when equilibrium dialysis is used.

Each test well in the plate consists of two chambers separated by a regenerated cellulose membrane with a molecular weight cut-off of either 5000 or 10000 Daltons. Each chamber holds up to 300 µL of sample or buffer.

To decrease the dialysis time, the plate needs to be rotated through 360° in a vertical position. Harvard Apparatus can also supply a 1- or 2-plate rotator or an 8-plate rotator with temperature control.

The 96-well plate was designed with SBS footprint and well spacing to meet automation needs.

Online: www.sdr.com.au
Phone: 02 9882 2882
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