Imaging flow cytometer

Amnis has released an Extended Depth of Field (EDF) upgrade for its ImageStream imaging flow cytometer.

The system provides researchers with numeric data from fluorescent signals on cells, as well as visual images of the cells showing exactly where the fluorescent signal is located. The system is suitable for applications including translocation experiments, localisation experiments, cell-cell interaction, signalling analysis, or any cellular experiment that requires image-based multi-parametric analysis that can be run in flow.

The EDF option provides high image quality across the entire focus range. Benefits include improved precision, enhanced cell feature discrimination, increased resolution and faster data acquisition. It also opens the system up to more application possibilities, such as fluorescent in situ hybridisation in suspension (FISH).

Counting FISH spots accurately requires all spots to be in focus. Since spots may be located anywhere in the nucleus, maintaining focus throughout the cell is critical. EDF technology expands the depth of focus over the entire cell to make FISH in flow possible.