siTOOLs Biotech siPOOLs (siRNA pools)

Short interfering RNA pools (siPOOLs) from siTOOLs BioTech are high-complexity pools of 30 optimally designed siRNAs that efficiently remove off-target effects and thus improve results.

The ‘Pack Hunter’ (pooling) approach counters off-targeting of individual siRNAs by diluting their concentrations below thresholds that stimulate phenotypes, increasing on-target specificity. With proprietary design algorithms, siRNA sequences within siPOOLs are also optimised for maximal transcript coverage, efficient hybridisation and filtered against paralogues, enabling efficient and specific gene silencing.

siPOOLs are suitable for use against coding or long non-coding RNAs, selected isoforms or closely related genes, and can be applied in combination to study synergistic gene interactions. With defined siRNA sequences within the siPOOL, targeting can be optimised against specific transcript isoforms or closely related genes.

Proprietary siRNA design algorithms select the most potent siRNAs based on thermodynamic properties that favour guide strand loading into the RNA-induced silencing complex (RISC). siPOOLs are designed for maximum coverage of all targeted transcripts with high specificity.