siTOOLs Biotech siPOOLs (siRNA pools)

Friday, 05 July, 2019 | Supplied by: Sapphire Bioscience



Short interfering RNA pools (siPOOLs) from siTOOLs BioTech are high-complexity pools of 30 optimally designed siRNAs that efficiently remove off-target effects and thus improve results.

The ‘Pack Hunter’ (pooling) approach counters off-targeting of individual siRNAs by diluting their concentrations below thresholds that stimulate phenotypes, increasing on-target specificity. With proprietary design algorithms, siRNA sequences within siPOOLs are also optimised for maximal transcript coverage, efficient hybridisation and filtered against paralogues, enabling efficient and specific gene silencing.

siPOOLs are suitable for use against coding or long non-coding RNAs, selected isoforms or closely related genes, and can be applied in combination to study synergistic gene interactions. With defined siRNA sequences within the siPOOL, targeting can be optimised against specific transcript isoforms or closely related genes.

Proprietary siRNA design algorithms select the most potent siRNAs based on thermodynamic properties that favour guide strand loading into the RNA-induced silencing complex (RISC). siPOOLs are designed for maximum coverage of all targeted transcripts with high specificity.

Online: www.sapphirebioscience.com
Phone: 02 9698 2022
Related Products

Phase Genomics Hi-C proximity ligation kits

Phase Genomics applies Hi-C and other proximity-ligation methods to enable chromosome-scale...

Mercodia Total GIP Northern Lights (NL)-ELISA

Mercodia's Total GIP NL-ELISA provides a chemiluminescent method for the quantitative...

Merck Plug & Play Upstream Development Service

Merck has launched its Plug & Play Upstream Development Service to help emerging biotech and...


  • All content Copyright © 2019 Westwick-Farrow Pty Ltd