QIAGEN QIAseq Targeted DNA Pro Panels

QIAGEN says its QIAseq Targeted DNA Pro Panels are based on a chemistry that halves to six hours the time researchers need for library preparation of DNA samples. By eliminating time-consuming bead-purification steps, its ultrafast, optimised workflow is said to cut hands-on time by 50% and pipetting steps by 60%. The detection of genomic variants is thus accelerated and refined.

The panels provide ease of use, efficiency, and expanded, ultrasensitive variant analysis using integrated unique molecular indices (UMIs). They are powerful tools for detecting genetic variations — like somatic mutations, single nucleotide polymorphisms and copy number variations — and can be bought off the shelf or custom made.

Researchers can detect single-nucleotide variants (SNVs), insertion deletions (indels) and structural variants with breakpoints at the nucleotide level and spanning multiple exons. Libraries created using the panels can be multiplexed with other chemistries in the same sequencing run on any sequencer. QIAseq Targeted DNA Pro Analysis and QIAGEN CLC Genomics software simplify data analysis.

The panels are suitable for cancer research labs with short turnaround times. Instead of bead clean-ups, the library prep is enzymatically treated, so the number of starting molecules in a sample stays the same and the complexity of the library is optimal. This is useful in areas like minimal residual disease (MRD) monitoring or non-invasive prenatal testing (NIPT) where users have few starting template molecules.