Flow cytometric chromosome classification

Flow cytometric chromosome classification (flow karyotyping), has been routinely used in the analysis of mammalian karyotypes as well as to assess chromosomal abnormalities. Various chromosome isolation methods have been developed in the preparation of isolated chromosomes from mitotic cells for flow karyotyping or flow sorting.

To generate a good chromosome preparation with minimal debris formation and large numbers of individual chromosomes for a well-resolved flow karyotype, protocols have made use of stabilising agents such as divalent cations and cationic polyamines in the isolation buffer.

Univariate or bivariate flow karyotype analysis on the isolated chromosomes can be performed by labelling with one or two DNA specific fluorescent dyes. Highly purified fractions of chromosomes can then be isolated rapidly from the labelled chromosomes using a high performance cell sorter.