Flow cytometric chromosome classification
Friday, 15 May, 2009
Flow cytometric chromosome classification (flow karyotyping), has been routinely used in the analysis of mammalian karyotypes as well as to assess chromosomal abnormalities. Various chromosome isolation methods have been developed in the preparation of isolated chromosomes from mitotic cells for flow karyotyping or flow sorting.
To generate a good chromosome preparation with minimal debris formation and large numbers of individual chromosomes for a well-resolved flow karyotype, protocols have made use of stabilising agents such as divalent cations and cationic polyamines in the isolation buffer.
Univariate or bivariate flow karyotype analysis on the isolated chromosomes can be performed by labelling with one or two DNA specific fluorescent dyes. Highly purified fractions of chromosomes can then be isolated rapidly from the labelled chromosomes using a high performance cell sorter.
How UV/VIS spectroscopy can optimise measurement in the lab
UV/VIS spectroscopy is a powerful method used on a daily basis in many laboratories. It is easy...
[AMCA Impact Study] How AI is Reducing Safety Documentation Time by 90%
Discover how AMCA is reducing administration,...
Leveraging Adeno-Associated Viruses (AAVs) in therapeutic approaches for monogenic neurological diseases
Learn about key strategies and innovations in this...
