Fast centrifugal partition chromatograph

Friday, 11 November, 2005 | Supplied by: http://www.lasersan.com/


Centrifugal Partition Chromatography (CPC) is a separation method based on the liquid partitioning of compounds.

Two immiscible liquid phases are in contact in the chromatographical column. Due to the centrifugal force field, one phase stays in the column; the other mobile phase is pumped into the column. The compounds migrate through the column according to their partition coefficient k.

CPC has a wide range of applications as all organic and inorganic molecules can be processed. Usually, they are classified from the polar to non-polar.

The absence of solid support gives a choice of applications only linked to the used solvent system. Separation goes from organic to inorganic, non-polar to very polar compounds with no degradation of fragile biological molecules.

Natural products from plants, soils, marine origin including alkaloids, saponins, flavonoids, sugars, carotenoids, fatty acids, polyphenols like tannins can be separated; as can biological molecules, including ADN, peptides, proteins and antibodies. Fermentation products; antibiotics; synthetic drug compounds; pesticides; and weed killers can also be separated.

FCPC Kromaton Centrifugal Partition Chromatography equipment are mono-axial and equipped with two high pressure rotary seals.

The units are suitable for fractionation and isolation of natural and synthesised compounds (organics or inorganics) for the use of biological tests of screening, or production of high value compounds. Quiet and compact, the system is especially useful in laboratories which are looking for an improved technique of extraction, concentration and purification.

The instrument is a benchtop chromatograph with a stacked circular disk-type column made of a succession of small loculi linked in cascade. The original design of the cells and the applied rotation secure: a good retention of the stationary phase while the mobile phase is pumped through; and a good dispersion of the mobile phase in each cell, which permits a great mass transfer between the two phases.

Two different models are available: Bench scale FCPC from analytical to preparative purification (three columns available: 100, 200, 1000 mL); and Pilot scale FCPC for production (5 L column).

The preparative chromatography technique is suitable for mg to g loadings and for milligram to > gram loadings. No solid support is required. Existing preparative HPLC equipment is used and the FCPC system simply replaces the column. The liquid mobile phase is pumped through the liquid stationary phase and compounds are separated by differences in partition coefficients. There is 100% recovery of samples and no irreversible adsorption.

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