JASCO infrared microscope

Tuesday, 30 June, 2015 | Supplied by: ATA Scientific Pty Ltd


Ftir

The JASCO infrared (IR) microscope is a powerful tool for obtaining IR images in order to analyse molecular structure. It is an alternative to methods for evaluating protein secondary structure such as X-ray crystallography, NMR and circular dichroism, which can only estimate the secondary structure of pure proteins.

While conventional IR microscopes use a single-channel detector to obtain IR imaging data, JASCO has developed a multichannel IR microscope with a built-in multichannel detector, rapid scan and high-performance sample stage which have enabled reduced measurement times. In addition, the system can be used to estimate the secondary structure of proteins.

The FTIR system allows measurements without extensive sample preparation and data can be collected on purified proteins or impure or unrefined protein. The estimation of protein secondary structure using unrefined materials is suitable for the fields of pathology and food preparation. No staining is required to obtain the images.

FTIR can be applied not only for proteins but also for estimating fatty acid and carbohydrate content. The JASCO Secondary Structure Estimation (SSE) software, in combination with principle component analysis, uses the IR spectrum to predict secondary structure within seconds while accounting for the various matrices (solvents, buffers, etc).

The microscope system is a fast analysis tool that enables the user to obtain IR images quickly and correlate functional group distribution and secondary structure of proteins. The system can be applied to various fields such as pathology, pharmaceutical, food science and more.

Online: www.atascientific.com.au
Phone: 02 9541 3500
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