Logos Biosystems X-CLARITY for fast and deep clearing of whole tissue and organs
Tissue clearing techniques have enabled the acquisition of high-resolution volumetric images without the need to reduce samples to thin serial sections. They offer a valuable way for examining the expression of molecular pathways in whole tissues and organs. X-CLARITY is becoming increasingly essential for light sheet microscopy. Fortunately, scientists no longer undertake the early tissue clearing methods that employed organic solvents, several of which were toxic, causing damage to delicate structures and quenching endogenous fluorescent proteins (FPs).
The X-CLARITY system and reagents offer a stress-free, step-by-step approach for tissue clearing. Based on the CLARITY principle, the method allows users to standardise, simplify and accelerate each step of the tissue clearing process. A whole mouse brain takes just 6 h to clear and endogenous FP signals are preserved.
Applications for the X-CLARITY method extend beyond brains: virtually any organ can be cleared, even organoids. The system efficiently preserves the 3D arrangement of cells and anatomical structures, proteins and nucleic acids, to enable volumetric imaging of large samples at single cell resolution.
The main components of the X-CLARITY tissue clearing system are as follows: the polymeriser sets the infused hydrogel, bolstering the tissue structural components; electrophoretic tissue clearing (ETC) chamber lipids are extracted actively through electrophoresis or passively, leaving behind a stable and transparent tissue–hydrogel hybrid that is chemically accessible for molecular phenotyping; and the control tower with built-in cooling system enables efficient tissue clearing.
Post clearing treatment, a transparent sample can be labelled with antibodies with the help from the DeepLabel Antibody Staining Kit, which enhances antibody penetration deep into clarified tissues, as well as the X-CLARITY Mounting Solution — a refractive index matching solution (RIMS) used prior to imaging. This reduces light scatter, which in turn increases optical transparency and consequently increases image quality and imaging depth.
Clearing is a valuable starting point for tissue imaging, but to make out fine molecular details, scientists need the means to ‘zoom in’. A range of solutions for rapid high content image acquisition and analysis are available.
For more information: https://www.atascientific.com.au/products/logos-biosystems-x-clarity/.
Phone: 02 9541 3500
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