Luxendo InVi SPIM AIM lattice light-sheet microscope for live cell imaging

Tuesday, 01 October, 2019 | Supplied by: SciTech Pty Ltd


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The Luxendo InVi SPIM AIM lattice light-sheet microscope features an advanced illumination module (AIM) for low-phototoxicity light-sheet fluorescence microscopy of live samples.

Leveraging the general benefits of single-plane illumination microscopy (SPIM) with high photon efficiency and short illumination times, the module enables the user to interactively customise the light-sheet shape to tailor the system to a particular specimen’s requirements. A variety of illumination patterns, including single or multiple variable Bessel beams, lattice light sheets and structured illumination, provides a range of single-instrument research possibilities for rapid, high-resolution 3D imaging of living cells.

The Luxendo InVi SPIM AIM lattice light-sheet microscope uses the high-performance InVi platform for gentle long-term imaging with precise control of physiological conditions. It has been designed to combine the advantages of several different illumination approaches to deliver the flexibility required to optimise bioimaging experiments, from a large field of view and high temporal sampling to spatial resolution at the physical limit.

The AIM expands the capabilities of the InVi SPIM systems with a choice for the optimal light-sheet for the experimental requirements, which is said to lead to better resolved images. This next-generation, lattice light-sheet microscope gives researchers the freedom to customise the light sheet and tune the microscope to a specific biological application, providing advantages in live-cell investigations with higher resolution.

The system maintains the ease of use and stability of the InVi SPIM, while enabling illumination of the sample with flexible light-sheet patterns. These patterns include the classical static Gaussian light-sheet and the scanned Gaussian beam, as well as sophisticated illumination schemes like Bessel beams or lattice light-sheets, improving the microscope’s resolution in time and space, while minimising phototoxic effects. The light-sheet geometry can be adapted to the sample in an easy, completely computer-controlled manner to offer high-resolution imaging in a user-friendly framework.

For more information: https://luxendo.eu/products/invi-spim/.

Online: www.scitech.com.au
Phone: 03 9480 4999
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