RedShiftBio Aurora TX for understanding RNA structure and stability

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Understanding RNA structure and stability is crucial not only for decoding its biological function but also for advancing RNA-based therapeutics and identifying novel drug targets. As interest in RNA-based treatments continues to grow, so does the need for analytical tools that can precisely characterise RNA structural dynamics. The Aurora TX system, powered by RedShiftBio’s microfluidic modulation spectroscopy (MMS) technology, seeks to redefine how researchers characterise RNA by offering greater insight into its structural transitions in real time.

MMS is a next-generation infrared spectroscopy technique capable of detecting subtle changes in RNA conformation and hydrogen bonding patterns. Using the Aurora TX system, MMS can analyse RNA structural elements, including base pairing, secondary structures like i-motifs and G-quadruplexes, and thermal melting dynamics. Additionally, MMS proves effective in detecting ligand-induced RNA conformational changes including structural differences in the payload upon LNP encapsulation and RNA-to-LNP ratio, showcasing its potential as a powerful tool for RNA-targeted drug discovery.

Aurora TX is designed for flexibility and precision across a wide range of biomolecules. With background-subtracted measurements, it works in native formulation buffers without requiring dilution or complex sample preparation and operates over a broad concentration range from 0.1 mg/mL to >200 mg/mL. This level of accuracy and versatility, coupled with an easy, automated workflow, utilisation of 96-well plates and fully integrated analytical software, addresses the limitations of existing technologies such as CD and FTIR.

The integration of thermal ramping in Aurora TX adds an extra dimension to biomolecular analysis by enabling researchers to induce high-resolution temperature stress, which should facilitate the detection of structural changes with precision and repeatability.

The system empowers researchers to delve deep into the characterisation of nucleic acids, proteins and other biomolecules without the need for spectroscopy expertise. With the ability to compare higher-order structures, stability profiles and similarity profiles, it facilitates informed decision-making across various stages of biopharmaceutical drug development, from discovery to quality control.

For more information or sample analysis, contact ATA Scientific or visit https://www.atascientific.com.au/products/redshiftbio-aurora-tx/.