Tomocube HT-2 Holotomographic microscope with 3D fluorescence imaging

Thursday, 01 November, 2018 | Supplied by: SciTech Pty Ltd


Tomocube ht 2 microscope

The HT-2 from Tomocube combines both holotomography and 3D fluorescence imaging into one microscope. The HT-2 facilitates three-dimensional fluorescence and optical diffraction tomography of live cells using structured illumination with minimal stress on cells. This technology is said to open the door to a variety of previously unexplored applications in the fields of bioscience and life science, helping researchers and clinicians better understand, diagnose and treat disease.

The HT-2 is able to achieve label-free imaging of cells and allows users to conduct long-term tracking of specific areas within live cells. It has the capacity to deliver correlative analysis in 2D, 3D and 4D with HT and fluorescence images, and incorporates a customisable three-channel LED light source (385, 470 and 570 nm). HT-2 generates Z-stack images with a motorised Z-drive (step resolution: 150 nm).

TomoStudio, the HT series operating software, controls the system and visualises the captured image in a multitude of different ways. This software enables fast image acquisition (holotomographic images captured at 2.5 fps), holographic staining and in-depth data analysis.

The product offers correlative microscopy in one instrument, providing high-quality 3D images of both holotomography and 3D fluorescence for each sample. Quantitative data is marked with fluorescence — the HT-2 provides morphological (volume, surface area, projection area, sphericity and ellipticity), chemical (dry mass and concentration) and mechanical (cell deformability) properties of cells with a 3D refractive index (RI) tomogram. Fluorescence imaging provides information about molecular specificity.

The simultaneous measurement capability of time-lapse 3D RI tomography and fluorescence imaging allows long-time tracking of specific targets in live cells. The fluorescence image provides the position of specific target organelles or structures in live cells, and consecutive measurements of time-lapse 3D RI tomography enable the monitoring of cells and their structures with minimal stress.

For more information: http://scitech.com.au/microscopy-systems/3d-holographic-microscopy/.

Online: www.scitech.com.au
Phone: 03 9480 4999
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