Cairn Research MultiSplit four-way image splitter

The MultiSplit from Cairn Research is a four-way image splitting device dividing an image into two, three or four separate spatially equivalent components which can be displayed side by side on a single camera chip.

Splitting is usually performed on the basis of wavelength and/or polarisation, allowing applications where there is a requirement for simultaneous or high-speed acquisition of multiple image emission bands or polarisation states. The simultaneous acquisition of up to four images offers a benefit over manual or electronic filter changers, as there is no longer a need to pause acquisition while the filter position is changed. This allows the user’s camera to be operated in high-speed stream modes and reduces demands on the software.

Applications include: simultaneous multidepth imaging (using independent lenses); Förster resonance energy transfer (FRET); ratiometric calcium, voltage and pH imaging; simultaneous multifluorescent probe imaging; polarisation studies (anisotropy); spinning disk confocal; super-resolution STORM/PALM/SIM; 3D super-resolution PALM/STORM (using cylindrical lenses); and simultaneous phase contrast/DIC and fluorescence.