Dionex Application Note 245 dye determination

Dyes have many applications in the food and beverage industries. Only a few have been approved for use in foods and beverages. Reversed-phase chromatography is a suitable technique for the analysis of dyes. Many dyes are hydrophobic, readily soluble in reversed-phase eluents and have strong visible and UV absorbance properties.

Application Note 245 (AN245) demonstrates fast separation of 10 dyes in less than 5 min using a Dionex Acclaim PA2 column. This method was used to determine the quantity of food dyes in six soft drinks and a gelatin dessert. The combination of a Dionex UltiMate 3000 Rapid Separation LC (RSLC) system and an Acclaim PA2 column is suitable for the fast analysis of food and beverage samples that have both approved and illegal dyes.

Application Note 245 is available at http://www.dionex.com/en-us/webdocs/85583-AN245-LC-Dyes-24Feb2010-LPN2385.pdf.