Dionex application note: determination of water-/fat-soluble vitamins

Thursday, 07 October, 2010 | Supplied by: Dionex Pty Ltd



Reversed-phase HPLC is a technique well suited to vitamin analysis. In Application Note 251 (AN 251), integrated and efficient methods for determining water- and fat-soluble vitamins in nutritional supplements were developed. Efficient and simple methods for the extraction and determination of the water- and fat-soluble vitamins were established and the separations were performed on the Dionex UltiMate HPLC system with Acclaim columns.

The water-soluble vitamins were well resolved on the Acclaim Polar Advantage (PA) II column using a phosphate buffer in the mobile phase. The fat-soluble vitamins were well resolved on the Acclaim 120 C18 column using an organic-solvent mobile phase. Method performance, including linearity, detection limits, reproducibility and analyte recovery, was evaluated for both methods. Five nutritional supplement samples were analysed and the results were equivalent to the labelled values.

Application Note 251 is available at http://www.dionex.com/en-us/webdocs/87181-AN251-HPLC-vitamin-supplements-25June2010-LPN2533.pdf.

Online: www.dionex.com
Phone: 02 9420 5233
Related Products

JASCO spectroscopy and chromatography instrumentation

JASCO is a provider of high-quality spectroscopy and chromatography instrumentation for...

Thermo Scientific FAIMS Pro Duo interface

The Thermo Scientific FAIMS Pro Duo interface supports biological mass spectrometry (MS) and...

Thermo Scientific Orbitrap IQ-X Tribrid mass spectrometer

The Thermo Scientific Orbitrap IQ-X Tribrid mass spectrometer has been designed specifically for...


  • All content Copyright © 2021 Westwick-Farrow Pty Ltd