Dionex application note: determination of water-/fat-soluble vitamins

Reversed-phase HPLC is a technique well suited to vitamin analysis. In Application Note 251 (AN 251), integrated and efficient methods for determining water- and fat-soluble vitamins in nutritional supplements were developed. Efficient and simple methods for the extraction and determination of the water- and fat-soluble vitamins were established and the separations were performed on the Dionex UltiMate HPLC system with Acclaim columns.

The water-soluble vitamins were well resolved on the Acclaim Polar Advantage (PA) II column using a phosphate buffer in the mobile phase. The fat-soluble vitamins were well resolved on the Acclaim 120 C18 column using an organic-solvent mobile phase. Method performance, including linearity, detection limits, reproducibility and analyte recovery, was evaluated for both methods. Five nutritional supplement samples were analysed and the results were equivalent to the labelled values.

Application Note 251 is available at http://www.dionex.com/en-us/webdocs/87181-AN251-HPLC-vitamin-supplements-25June2010-LPN2533.pdf.