Refeyn MassFluidix HC microfluidic system

Refeyn has unveiled the MassFluidix High Concentration (HC) microfluidic system, which extends the analytical strength of mass photometry to high-concentration protein samples. Compatible with Refeyn’s Two^MP mass photometers, the system enables measurement of low-affinity biomolecular interactions through rapid dilution, such that the interaction state at micromolar concentrations is captured before their equilibrium is disrupted.

Researchers can now analyse samples with concentrations of up to 90 µM, a 100-fold increase on previous capabilities, the company claims. This approach builds on the speed and simplicity already offered by mass photometry and opens up applications such as the study of low-affinity protein-protein interactions, transient binding and oligomerisation — all label-free, in solution and at the single-molecule level.

Designed for easy and intuitive operation, the system is designed to provide precise and rapid dilution of up to 10,000-fold in milliseconds, facilitating the detection of low-affinity complexes that have not previously been observed. The system consists of two parts: the microfluidics device and a consumable chip for immediate analysis allowing the sample to reach the observation window within <50 ms of the beginning of the dilution process.

According to Refeyn, mass photometry offers a fast and precise approach to measuring molecular mass distribution at the single-molecule level, providing researchers with a powerful tool for understanding complex biological systems. For researchers looking to expand the potential of mass photometry and streamline their protein analysis workflows, the MassFluidix HC system is a useful addition to their laboratory.