Refeyn SamuxMP Auto mass photometer for automated AAV capsid analysis

Refeyn has announced its Samux^MP Auto mass photometer, designed to reduce manual operation time while increasing reproducibility in adeno-associated virus (AAV) characterisation. The benchtop automated mass photometer addresses a major bottleneck in AAV development and manufacturing: the need to efficiently characterise samples at multiple stages throughout bioprocessing. It autonomously determines AAV sample purity, aggregation and empty/full ratios, enabling scientists to save time and effort in their workflows.

Mass photometry is a bioanalytical technology that enables the measurement of mass at the single-particle level by quantifying light scattering from biomolecules and other particles in solution at the benchtop. The technology can detect rare species in a heterogeneous sample and is serotype-agnostic, providing advantages over other methods of AAV characterisation that require more investment, space, sample, time and expertise to generate equivalent data.

The product is said to deliver precise characterisation of up to 24 samples in as little as 90 min, without the need for operator supervision or input during that time. Current SamuxMP users can also augment their system with the robotics unit. This enables them to improve their workflows and enhance their productivity while benefiting from reduced room for human error and the ability to standardise workflows for more consistent measurements.

The solution consists of a Samux^MP mass photometer integrated with a liquid handling robotics unit and Refeyn’s Acquire^MP and Discover^MP software. These work together to provide an easy-to-use automated system, requiring as little as one day of training to operate. The result is quick AAV characterisation, with automatic calculation of empty, filled, partially filled and overfilled capsids. Refeyn has also introduced a titre estimation capability to the Samux^MP range: both instruments can now provide an estimate of the concentration of AAV capsids in a sample as part of the standard data analysis procedure.