Scientifica HyperScope multiphoton imaging system

Scientifica’s Hyperscope is a multiphoton imaging system that enables simultaneous two-photon microscopy and photoactivation in either in vitro or in vivo samples.

The system has a dual-scan head to allow two identical light paths to be manipulated through the microscope. This enables simultaneous two-photon imaging and photoactivation with good performance.

With multiple scanning options, the scan mirrors on the imaging path can be arranged in multiple configurations depending on the user’s requirements. With three mirrors in series, the user has the option of galvo, resonant or galvo/resonant arrangement.

The optics are coated for wavelengths of 700 to 1400 nm to allow excitation dyes further into the infrared spectrum and potentially perform three-photon imaging. This allows greater flexibility for multiphoton imaging and the ability to image deeper into the sample.

The microscope can be set up with or without substage optics for in vitro or in vivo samples respectively. For larger in vivo specimens and more elaborate experimental layouts, an extended scan head can be used.