SGE ProteCol HPLC column range

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The metal-free flow path and large pore sizes in the SGE ProteCol HPLC range offer solutions to two problems faced in life sciences - working with metal chelating compounds or separating large proteins such as intact membrane proteins.

The columns solve the problem of separating chelating compounds, such as natural products, antibiotics and antifungals, with minimal preparation time.

The columns have an inert metal-free flow path featuring ultrapure silica, inert PEEK-lined column hardware, PEEK frits and fully end-capped optimised C18 phase. The inert flow path substantially reduces sample and column preparation time and improves peak shape, resulting in improved reproducibility and sensitivity.

There is also the benefit of fewer artefacts, due to reduced carryover, and the column provides the ability to use MS, ELSD and Corona CDA techniques.

The columns solve the problem of separating proteins, including membrane proteins, due to a combination of features - 1000 Å pore size, intermediate polarity C8 phase and inert glass-lined column hardware. These combined features enable continuity of HPLC for all protein separation needs, providing a simplified workflow, facilitating the use of MS and potentially eliminating SDS-PAGE.