Teledyne Photometrics Evolve EMCCD cameras

Teledyne Photometrics’ Evolve is a high-resolution, back-illuminated EMCCD camera providing high sensitivity for low-light applications. The series features highly stabilised deep cooling and back-illumination for >95% quantum efficiency, as well as electron multiplication to achieve a read noise as low as 0.25e- without sacrificing frame rate. For single-molecule imaging, TIRF microscopy and more, users are able to achieve high imaging performance when every photon counts.

The range includes 13 and 16 μm pixel cameras to provide optimum sensitivity. However, resolution was previously limited for EMCCDs due to a lack of smaller pixel size options. Now with sensor manufacture taking place in-house, Teledyne is able to offer a 10 μm pixel back-illuminated EMCCD, matching the required pixel size for 100x oil immersion microscope objectives. Full-frame imaging rates of up to 61 fps can be sustained in high-speed readout mode, with frame rates greater than 4000 fps available when imaging with regions of interest.

Through their single output amplifier, EMCCDs offer uniform noise performance across the sensor. This is advantageous for analysis in many single-molecule fluorescence imaging techniques. The series fine tunes the performance of EMCCD sensors to provide high image quality through careful electronic design and calibration.