Thermo Scientific MSIA Streptavidin EVO microcolumns

Tuesday, 23 February, 2016 | Supplied by: Thermo Fisher Scientific

A fully automated affinity purification system is designed to enable scientists to analyse large biomolecules, such as proteins and antibodies, with improved accuracy, consistency and simplicity, even at high throughputs.

The Thermo Scientific MSIA Strepdavidin EVO microcolumns have been designed to bring the novel mass spectrometric immunoassay (MSIA) affinity purification technology to Tecan’s Freedom EVO series of robotic platforms that are equipped with a 96-multichannel arm (MCA96). When combined with the platform, the microcolumns enable fast and accurate isolation of target analytes from complex biological matrices.

Housed within a pipette tip, the monolithic columns are densely coated with streptavidin for analytical affinity purification of any biotinylated affinity ligand, even at low concentration. Since the technology is incorporated into a pipette tip, users can analyse sample volumes as low as 10 µL. When compared to traditional resin and bead-based methods, MSIA technology is said to provide lower background noise and improved analytical sensitivity, while the simple workflow enables easier method standardisation and transfer between labs.

The microcolumn is suitable for biologics and biosimilars discovery and development. It is particularly suited to laboratories performing large molecule bioanalyses that demand high-quality, reproducible and unambiguous results with minimal background noise. The product is designed to help scientists save both precious samples and time, providing fast assay development, scalable sample processing and surety of data quality and consistency.

Phone: 1300 735 292
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