Wyatt Technology SEC columns for protein analysis

Thursday, 21 January, 2010 | Supplied by: Shimadzu Scientific Instruments (Oceania) Pty Ltd


Wyatt Technology’s size exclusion chromatography (SEC) columns for protein analysis by multi-angle light scattering have been expressly designed to achieve the best resolution and reproducibility as well as the maximum detection sensitivity for protein characterisation.

Multi-angle light scattering has become an invaluable tool for protein characterisation. The combined SEC-MALS method has many applications, including quantification of protein aggregation, determination of protein conjugate stoichiometry and confirmation of the oligomeric state of a protein.

Traditional SEC columns used for protein separation often shed particles, some for an extended period of time. As a result, SEC-MALS methods require excessive downtime to condition columns in order to avoid compromising data quality. The latest Wyatt Technology SEC columns cease shedding particles after a very short time and produce low light scattering baseline noise and good pressure shock resistance.

The SEC columns have very good resolution and reproducibility as well as an extended applicability for proteins classes including membrane proteins, owing to the use of high-quality silica with well-controlled surface chemistry and manufacturing processes. The columns also have a full range of pore size selections to choose from.

Online: www.shimadzu.com.au
Phone: 02 9684 4200
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