AdipoGen n-Octyl-β-D-glucopyranoside (ultrapure) non-ionic detergent

AdipoGen’s n-Octyl-β-D-glucopyranoside (ultrapure) is a non-ionic detergent for the functional solubilisation, isolation, purification and crystallography of membrane proteins. It is a mild, non-denaturing detergent that is used for the solubilisation and reconstitution of membrane-bound proteins in their native state and for the preparation of lipid vesicles.

Its well-defined chemical structure, small uniform micelles and high water solubility are said to offer the product advantages over most other non-ionic detergents for membrane solubilisation. The high critical micelle concentration (0.7%) of n-octylglucoside facilitates ready removal from final protein extracts by dialysis or gel filtration.

The detergent can be used in lysis and solubilisation buffers for bacterial lipoprotein preparations, mitochondrial extracts or for determination of protein content by BCA assay. It can be used in 2D electrophoresis, to improve selectivity of immunoprecipitation of phosphotyrosine-modified proteins and to increase the resolution of proteins in 2D gels, according to the company.