EXBIO ApoFlowEx FITC Kit for apoptosis detection

Apoptosis is a regulated cell death process characterised by morphological and biochemical features occurring at different stages. One of the earliest indications of apoptosis is the translocation of phosphatidylserine (PS) from the inner to the outer leaflet of the plasma membrane. Once exposed to the extracellular environment, PS becomes available for annexin V, a 35–36 kDa Ca2+ dependent phospholipid-binding protein with a high affinity for it. Detection of such membrane changes by Annexin V binding to PS has been suggested as a suitable assay for early apoptotic cells.

Early apoptotic cells still have an intact plasma membrane so their DNA is not accessible for propidium iodide dye, and these cells are annexin V-positive and propidium iodide-negative, unlike viable cells (annexin V-negative, propidium iodide-negative) and necrotic cells or late apoptotic cells (annexin V-positive, propidium iodide-positive).

EXBIO enables apoptosis detection via flow cytometry with the ApoFlowEx FITC Kit. The kit consists of fluorescently labelled annexin V and propidium iodine for flow cytometry identification and enables the differentiation of early apoptotic, necrotic and viable cells. It is available alongside other apoptosis detection solutions, including annexin V in multiple formats, annexin V binding buffer and antibodies to apoptosis-related antigens.