GeneTex EasyBlot reagents and kits

Immunoprecipitation (IP) and co-immunoprecipitation (co-IP) assays often necessitate the use of the same antibody, or antibodies from the same host, during both the IP and western blot (WB) procedures. Denatured immunoglobulin (Ig) in the IP sample can be bound by the secondary antibody used for the WB. Thus, depending on the molecular weight of the target protein, the desired band may be partially or completely obscured on the WB image by signal from denatured heavy (-50 kDa) and/or light (-25 kDa) Ig chains.

GeneTex’s EasyBlot secondary antibodies are specifically optimised to eliminate this problem. They bind only the native (non-denatured and non-reduced) primary antibody used for WB analysis and not the denatured IP antibody chains. In addition, the specially formulated EasyBlocker reagent will reduce background signal caused by free Protein A or Protein G.

The product efficiently eliminates denatured IgG masking of the target protein’s signal. It is suitable for WB following either IP or co-IP analysis using Protein A-, Protein G- or Agarose-conjugated antibodies.

A broad range of EasyBlot reagents is available for detecting various hosts: rabbit, mouse, rat, goat or sheep. It’s easy to switch from regular secondary antibodies to EasyBlot secondary antibodies.