Innova Biosciences antibody purification systems

Tuesday, 21 August, 2012 | Supplied by: http://www.innovabiosciences.com/images/stories/innova/antibody_purification_guide.pdf


Innova Biosciences has designed a range of accessory products which can be used to separate antibodies from unfavourable starting points. The kits are easy to use and are compatible with the Lightning-Link Antibody Labelling range. Combining an accessory kit with a Lightning-Link product, users are able to label an antibody irrespective of the antibody starting formulation.

The AbSelect range involves the capture of the antibody on a protein (A, G or anti-Mouse) that has been immobilised to agarose beads and can be used to purify IgG fractions. Unwanted substances are removed by incorporating simple wash steps, and the captured antibody is subsequently eluted.

AbSelect Antibody Purification System, AbSelect G Antibody Purification System and AbSelect Mouse Antibody Purification System readily remove unwanted substances such as proteins, preservatives, amino acids and other components that can interfere with labelling reactions. They can either be used to purify antibodies from crude samples (ascites fluid or serum) or poor buffer formulations (buffers containing high concentrations of BSA, azide or Tris). The antibody to be purified or cleaned is up to 0.5 mg and in a volume of 100 to 500 µL.

AbSelect Serum Purification Systems and AbSelect G Serum Purification Systems are developed for antibodies generated from ascites fluid and serums are often supplied as crude formulations. The serum system is used to purify up to 20 mg of antibody from these types of media.

AbSelect TCS System and AbSelect Mouse TCS System are developed for antibodies generated from hybridoma cell lines and supplied in tissue culture supernatant (TCS). The systems are used to purify antibodies from 10-50 mL of TCS.

The Antibody Concentration and Cleanup Kit allows for the quick and easy concentration of antibodies. The kit can also be used to reduce the concentration of any undesirable low molecular weight additives. The kit’s method uses a simple spin column to remove excess buffer from the antibody, providing a more concentrated antibody solution.

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