Enzo Life Sciences Mito-ID assays for mitochondrial function

Saturday, 18 October, 2014 | Supplied by: United Bioresearch Products Pty Ltd

Detecting changes in metabolism is crucial to the understanding of many pathological processes. Monitoring both oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) provides a comprehensive analysis of cellular bioenergetics. Enzo Life Sciences’ Mito-ID assays work together to assess the dynamic interaction between mitochondrial respiration and glycolytic flux.

Mito-ID Oxygen and pH Sensors are high-throughput assays with easy mix-and-measure protocols. The sensitive, reversible probes provide real-time analysis and are compatible with standard fluorescence plate readers.

Mito-ID Extracellular O2 Sensor Kits are oxygen-sensitive phosphorescent probes which can be used to assess O2 consumption by cultured cells, isolated mitochondria, microorganisms, tissues and enzymes. The probe increases in signal intensity with O2 consumption. It is amenable to 96- and 384-well microplates for high-throughput analysis on standard fluorescence plate readers (Ex/Em 380/650 nm). A cell-permeable probe is also available for tracing intracellular O2 levels, and the user may multiplex with the Mito-ID pH sensor probe to confirm mitochondrial toxicity.

The Mito-ID Extracellular pH Sensor Probe is a pH-sensitive phosphorescent probe which can be used to monitor cellular acid extrusion - the result of increased glycolytic flux associated with mitotoxicity. The probe increases in signal intensity with increasing acidity. It provides a simple mix-and-read protocol for 96-well microplates and is amenable to standard fluorescence plate readers (Ex/Em 340/615 nm).

The Mito-ID Membrane Potential Cytotoxicity Assay Kit is a real-time mitochondrial membrane potential assay, said to be 10x more sensitive than JC-1 with good aqueous solubility. The product features photostable dual-emission dye and no-wash/no-medium removal. It is suitable for high-throughput applications; separate Mito-ID Red/Green assays are available for the detection of mitochondrial mass.

Online: www.unitedbioresearch.com.au
Phone: 02 4575 0309
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