Cayman Chemical 2′3′-cGAMP ELISA Kit

Sensitive and direct measurement of cGAMP levels in cell lysates is now possible with Cayman’s 2′3′-cGAMP ELISA Kit. Developed in conjunction with expert nucleotide scientists at Biolog Life Science Institute, the assay is designed to provide key improvements from the previous methods used to quantify 2′3′-cGAMP content and offers good sensitivity in the low pg/mL range.

2′3′-cGAMP acts as a second messenger during host defence and may also have roles in autoimmune or inflammatory diseases, including cancer. It is produced when the DNA sensor, cyclic GMP-AMP synthase (cGAS), detects the presence of nucleic acids in the cytosol of mammalian.

2′3′-cGAMP binds tightly to the adaptor protein STING (stimulator of interferon genes), which initiates the recruitment and activation of downstream proteins (TBK1 and IRF3) that induce the transcription and translation of type I interferon (IFN), a potent antiviral cytokine. Modulation of cGAS activity with subsequent induction or inhibition of 2′3′-cGAMP formation is an active target of pharmacological intervention for cancer immunotherapies and autoimmune therapies.

By enabling sensitive quantification of cGAMP levels in cells, Cayman’s 2′3′-cGAMP ELISA Kit can be used to assess the effectiveness of novel inhibitors in preclinical studies. The product is also useful for nonclinical users needing quantify 2′3′-cGAMP content, since the assay is suitable for monitoring the kinetics of 2′3′-cGAMP formation and hydrolysis in a biological setting.

Along with cyclic dinucleotides, recombinant STING variants and other key targets, the assay kit fits within a suite of tools available from Cayman to study the cGAS-STING-IFN signalling axis.