DyNAbind DNA-encoded library (DEL) kits

DyNAbind’s fragment-based DNA-encoded library (DEL) kit, containing more than 370,000 paired arrangements of molecular fragments, is designed to provide improved signal-to-noise ratios and reduced false positive hit rates, precluding lengthy hit validation steps and allowing for meaningful results in days instead of months. The kit also includes customer access to a secure analysis portal. Hosted by Merck, this portal enables deconvolution of the DNA sequence to reveal the chemical identity of the fragment or fragment-pair hits.

Unlike traditional high-throughput screening methods which require sophisticated robotics equipment and specialised biochemical assays, DELs offer a low-footprint solution that can be used in any laboratory with regular molecular biology equipment.

DyNAbind’s Dynamic Libraries are built on a novel DNA architecture that allows the formation of randomised, transient pairs of chemical structures. These pairs reshuffle in solution until stabilised by binding to the target protein, offering in-situ generation of additional copies of strongly binding pairs for good signal-to-noise ratio and reduced false positive hits.

The chemical structures available in the kit have been carefully selected to ensure maximum diversity and medicinal chemistry relevance. Before being integrated into the library, each molecule was cross-checked to prevent the inclusion of toxic or promiscuously binding substructures, while strict cut-offs were applied to physicochemical properties.

The fragment-based technology provides both an easy access point to DEL technology for beginners and value to groups already engaged in traditional DEL technology by accessing a different structural type of potential hits.