Merck Millipore protocol for purification of recombinant proteins

Friday, 04 April, 2014 | Supplied by: Merck Pty Ltd

Merck Millipore has introduced a recombinant protein purification protocol that combines lysis and purification into a single step. The condensed workflow can be completed in about 45 min, which is said to reduce processing time by 75% compared to traditional techniques while delivering high yield and purity.

The protocol is claimed to require less hands-on time than the conventional workflow, providing greater consistency and results in more reproducible yields. The method uses a specialised lysis buffer in combination with magnetic affinity capture beads, which together eliminate the need to clarify lysates by centrifugation. As a result, the workflow can be automated using a high-throughput particle processor, further reducing sample variability.

The one-step lysis and purification protocol has been validated specifically for Merck Millipore’s PureProteome Nickel Magnetic Beads, optimised for the capture of histidine-tagged proteins, and Merck Millipore’s BugBuster Master Mix, a lysis reagent that allows for non-mechanical extraction of soluble protein from bacterial cells. The reagent combines detergent-based lysis with enzymatic agents, Benzonase nuclease and rLysozyme enzyme in a ready-to-use formulation.

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