VisiTech International VT-iSIM multi-point super-resolution imaging system

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The VT-iSIM from VisiTech International is a multi-point super-resolution imaging system, enabling parallel processing of emitted photons through the emission micro-lens array optic and thus allowing super-resolution images to be collected at up to 200 fps at a pixel resolution of 1024 x 1024 and FOV of 66.56 x 66.56 µm. Spatial resolution at this rate of acquisition is maintained at 120 nm in X and Y and 300 nm in Z, without the requirement for additional magnification.

The system can image at spatial and temporal resolutions and does not require any specific fluorophores, immersion oils, objective lenses or microscope frames to work. If a sample fluoresces, the user can enhance their spatial and axial resolution by up to 2x regular for wide-field microscopy. VT-iSIM produces images beyond the diffraction limit in real time onto a 2D array detector such as an sCMOS camera with no post-processing required. With an sCMOS camera, the achievable capture rates are 200 fps @ 1024 x 1024, 400 fps @ 1024 x 512 and 800 fps @ 1024 x 256.

The product can chromatically split the fluorescence emission by wavelength onto multiple array detectors or even onto a single array detector to enable parallel multi-colour image acquisition at spatial resolutions of 120 x 120 x 300 and temporal resolutions of up to 1 KHz.

VT-iSIM uses multi-point confocal scanning to generate super-resolution images with low photo-bleaching, and the ability to image thicker samples such as tissue sections or whole animals. The system also features variable pin hole size selectable from 10–64 µm and an automated three-position dichroic changer. Other features include a regular or high-speed six-position emission filter changer and excitation is achievable with up to six SS lasers from visible range (405, 445, 488, 514, 532, 561, 642). Camera and excitation sync comes as standard. For the most accurate sampling, the camera must have pixel size <6.5 µm and the camera connection is via regular c-mount.

The VT-iSIM can be added to any regular epi-fluorescent microscope (upright or inverted, eg, Nikon, Olympus, Zeiss or Leica) to enable a path into high-speed super-resolution imaging. The upgrade can be performed onsite with other software platforms including VoxCell Scan, Meta-Morph and NIS Elements.