3M Harvest RC chromatographic clarifier
The 3M Harvest RC utilises fibrous anion exchange (AEX) chromatography to efficiently separate cells, cell debris and DNA from the harvest fluid containing the target product. Precision quaternary ammonium (Q) functionalised polypropylene fibre, combined with a 0.2 µm PES membrane, provides predictable clarification that is scalable from discovery to commercial manufacturing.
The product enables a single-stage clarification process of low- to high-density cell culture (> 40 million cells per mL) with high recovery, and high fidelity of soluble and insoluble contaminant separation. Cells are bound inside the media by electrostatic charge interaction with the AEX chromatographic fibres. This results in the efficient retention of large and small particulates without developing a surface cake layer. The media can also remove soluble impurities, which is said to result in cleaner effluent than centrifugation or depth filtration.
Phone: 13 00 367 362
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