Bio-Rad Laboratories Nuvia wPrime 2A Media chromatography resin

Bio-Rad Laboratories has announced its Nuvia wPrime 2A Media, a scalable weak anion exchange and hydrophobic interaction (AEX-HIC) mixed-mode chromatography resin for small- to large-scale biomolecule purification. The resin has been used to remove host cell proteins, high and low molecular weight aggregates and impurities, and DNA from monoclonal and bispecific antibodies, as well as adeno-associated virus.

Built upon Bio-Rad’s Nuvia bead technology, the product features a polyacrylamide (acrylamide polymer) base bead that can be used for laboratory- and small-scale purification workflows, as well as for pilot-scale bioproduction and manufacturing-scale downstream processing. Nuvia resins are designed to have minimal non-specific binding reactions, due to the hydrophilic nature of the bead polymer, and maintain a constant dynamic binding capacity over a wide range of flow rates. The bead allows for fast mass transfer, is chemically and mechanically stable over a wide range of conditions, and is easy to pack.

Developed in collaboration with Bio-Rad’s partners in industrial biotherapeutic production, the product provides a wide purification design space. The charge state of the resin’s functional ligand can be modulated by the pH of the buffer, enabling the purification of otherwise difficult-to-separate biomolecules from other impurities.