Cayman Hydrogen Peroxide Ratiometric MaxSpec Kit

The Cayman Hydrogen Peroxide Ratiometric MaxSpec Kit for mass spectrometry helps overcome the difficulties of directly measuring mitochondrial H₂O₂ in vivo.

The kit includes MitoB, MitoP and their respective deuterated standards (MitoB-d15 and MitoP-d15), plus a detailed protocol to determine the MitoP/MitoB ratio by LC-MS/MS in cell culture and animal models. MitoB (MitoBoronic Acid) is taken up specifically by polarised mitochondria and accumulates in the matrix. The selective reaction of the arylboronic acid moiety of MitoB with H₂O₂ forms the phenol product MitoP (MitoPhenol). Therefore, the increase in the MitoP/MitoB ratio over time indicates the level of mitochondrial H₂O₂. The ratio of MitoP/MitoB is measured in sample extracts by LC-MS/MS using deuterated internal standards to correct for extraction and detection variations.

Each compound in the kit is formulated as a ready-to-use, quantitative solution to streamline sample and standard preparation for mass spec analysis. These formulations are guaranteed to meet rigorous quality standards, ensuring consistency and reproducibility from run to run.